The monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes
| Target |
CD3 |
| Reactivity |
Human, Monkey |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
FCM |
| Recommended dilutions |
FCM: 20 µl/1 million cells. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Human leucocytes. |
| Purification |
Affinity Chromatography |
| Form |
Liquid |
| Isotype |
IgG1 |
| Conjugation |
OC-515 |
| Specificity |
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Storage |
Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light. |
| Molecular Weight |
22/26/30 kDa |
| Swiss Prot |
P07766
|
| GeneID |
916
|
| Buffer |
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 2-3 weeks. |
| Note |
This product is for research use only. |