SMPD2 Antibody is a Rabbit Polyclonal antibody against SMPD2. Sphingomyelinases (SMases) catalyze the hydrolysis of sphingomyelin to produce ceramide and phosphocholineCeramide is an important bioactive lipid triggering signal transduction involved in cell prolifeRation, apoptosis and differentiationA number of SMases have been described and categorized based on their optimum pH activity, cation dependence, tissue distribution, and subcellular localizationThese include a lysosomal acid SMase, a Zn++-dependent secreted acid SMase, a membrane-bound Mg++-dependent neutral SMase, a Mg++-independent neutral SMase, and an alkaline SMasenSMase1 (also termed SMPD2) is a Mg++-dependent neutral SMase that is widely expressed and predominantly localized to the endoplasmic reticulumThis protein has also been shown to have lyso-platelet activating factor (PAF) phospholipase C activityA second neutral SMase, nSMase2 (also termed SMPD3) is predominantly expressed in the brainThe activity of neutral SMases is regulated by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic stimuliAnalysis of single and double knockouts of the SMPD2 and SMPD3 has revealed that loss of both genes leads to complete loss of neutral SMase activity with developmental defects observed with loss of nSMase2.

Target	SMPD2
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	ELISA, WB, IHC
Recommended dilutions	WB: 1/500 - 1/2000, IHC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Recombinant protein of human SMPD2.
Purification	Affinity Chromatography.
Form	Liquid
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Swiss Prot	O60906
GeneID	6610
Gene Symbol	SMPD2
Buffer	PBS, pH7.3, containing 0.02% sodium azide and 50% Glycerol.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.