RAD17 Antibody is a Rabbit Polyclonal antibody against RAD17. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
| Target |
RAD17 |
| Reactivity |
Human |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Tested Applications |
WB, IF/ICC, IP |
| Recommended dilutions |
WB: 1/500 - 1/2000, IF/ICC: 1/10 - 1/100, IP: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Recombinant protein of human RAD17. |
| Purification |
Affinity purified. |
| Form |
Liquid |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
Calculated MW: 57 kDa/66 kDa/75 kDa/77 kDa
Observed MW: 85 kDa |
| Swiss Prot |
O75943
|
| GeneID |
5884
|
| Gene Symbol |
RAD17 |
| Concentration |
> 1 mg/ml |
| Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |