CD133 Antibody is a rabbit polyclonal antibody against human, mouse and rat CD133 (prominin-1). In humans, the CD133 glycoprotein is encoded by the PROM1 gene and is approximately 100-120 kDa in weight. This protein is a member of the pentaspan transmembrane glycoprotein (5-TM) family, which specifically localize to cellular protrusions. CD133 is expressed in a broad range of cell types including haematopoietic stem cells, mammary glands and cells of the digestive tract. Its expression is regulated by several different factors including TGF beta 1 and microRNA.

CD133 has also been identified as a key modulator of several different functions in cancer cells including tumorigenesis and metastasis which promotes their growth, spread and survival. As a result, CD133 is now frequently used as a biomarker for detecting and isolation stem cell and cancer cell populations. Abbexa's CD133 Antibody (abx019041) has been tested in several different applications including Western blotting (WB), immunohistochemistry, immunofluorescence (IF/ICC) and flow cytometry. This allows for the detection, observation and isolation of CD133 and CD133+ cells.

Target	CD133
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB, IHC, IF/ICC
Recommended dilutions	WB: 1/1000, IHC-P: 1/200, IF/ICC: 1/200, FCM: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	A synthetic peptide (conjugated with KLH) corresponding to near C-terminal of human CD133.
Purification	Immunogen affinity purified.
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Swiss Prot	O43490
GeneID	8842
Gene Symbol	PROM1
OMIM	604365
HGNC	9454
Ensembl	ENSG00000007062
Buffer	TBS, pH 7.4, 0.5% BSA, 40% glycerol and 0.05% sodium azide.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.