The monoclonal antibody is directed against the CD4-antigen (T4-antigen),which is expressed on human peripheral T lymphocytes and 80% of thymocytes and reacts on a low level with human monocytes and macrophages
| Target |
CD4 |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
FCM |
| Recommended dilutions |
FCM: 20 µl/1 million cells. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Japanese White Rabbit thymocytes. |
| Purification |
Affinity Chromatography |
| Form |
Liquid |
| Isotype |
IgG2a |
| Conjugation |
Biotin |
| Specificity |
60Kd MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD4 cluster and present on 54% of peripheral blood T lymphocytes, 50% of thymocytes and some malignant cells of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD4 antigen although cytoplasmic expression has been observed in monocytes/macrophages. The CD4 positive T lymphocyte subpopulation has been characterised functionally as comprising helper cells active in amplification of immune responses. |
| Storage |
Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light. |
| Molecular Weight |
60 kDa |
| Swiss Prot |
P01730
|
| GeneID |
920
|
| Buffer |
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 2-3 weeks. |
| Note |
This product is for research use only. |