Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs), 1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Target	USP29
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB, IHC
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	KLH-conjugated synthetic peptide between 660-690 amino acids from the Central region of human USP29.
Purification	Purified through a protein A column, followed by peptide affinity purification.
Form	Liquid
Isotype	IgG
Conjugation	Unconjugated
Storage	Store at 2-8°C for up to 2 weeks. Aliquot and store at -20 °C for long-term storage. Avoid repeated freeze/thaw cycles.
Swiss Prot	Q9HBJ7
Gene Symbol	USP29
Enzyme Commission Number	EC 3.4.19.12, EC 3.1.2.15
Buffer	PBS containing 0.09% sodium azide.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.