Vitamin D2 (VD2) Antibody Pair for use in Sandwich ELISA assay development. The 10 × 96 tests size contains:
- 400 μg Vitamin D2 (VD2) rabbit polyclonal capture antibody,
- 100 μg Vitamin D2 (VD2) Biotinylated Competitor,
- 10 μg Vitamin D2 (VD2) standard.
Target |
Vitamin D2 (VD2) |
Reactivity |
Human |
Tested Applications |
ELISA |
Recommended dilutions |
Dilute the Capture Antibody 125-fold with Coating Buffer. Dilute the Biotinylated Competitor 200-fold with Detection Antibody Diluent. Optimal dilutions/concentrations should be determined by the end user. |
Form |
Liquid (Capture Antibody and Detection Antibody) |
Reconstitution |
Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user. |
Test Range |
1.23 ng/ml - 100 ng/ml |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Concentration |
400 µg / 0.8ml (Capture Antibody), 100 µg / 0.5 ml (Biotinylated Competitor), 10 µg/vial (Standard) |
Standard Form |
Lyophilized |
ELISA Type |
Sandwich |
Capture Antibody Host |
Rabbit |
Capture Antibody Clonality |
Polyclonal |
Capture Antibody Conjugation |
Unconjugated |
Detection Antibody Conjugation |
Biotin |
Buffer |
The capture and detection antibody both contain 0.1% sodium azide. |
Availability |
Shipped within 5-20 working days. |
Note |
This product is for research use only. |