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| Product Name | Cas9 (D10A), Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pyogenes |
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| Description | Purity ~95% (SDS-PAGE). The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease activity. To improve the off-target mutagenic effects of this system, the Cas9 Nickase D10A NLS Protein was developed with a D10A mutation in its RuvC1 nuclease domain with an SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. This mutant form results in the generation of single strand nick instead of a double stranded break (DSB), like that generated by the Cas9 Nuclease, at the target site. Since a single strand break, or nick, is normally quickly repaired through the homology directed repair pathway using the intact complementary DNA strand as the repair template, off-target effects of the Cas9 Nickase is minimized. To utilize Cas9 Ni |
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| Size | 50pmol, 250pmol |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Cas9 (D10A), Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pyogenes (CRISPR Associated Protein 9) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 500556 |
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| Order / More Info | Cas9 (D10A), Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pyogenes from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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