| 产品详情 |
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| Antigenic Specificity | TLR9 |
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| Clone | 5G5 |
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| Host Species | Mouse |
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| Reactive Species | mouse, canine |
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| Isotype | IgG2a |
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| Format | Protein A/G purified |
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| Size | 0.05 mg |
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| Concentration | 0.1 mg/ml |
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| Applications | Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. IHC-Frozen: 10um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. IF: cells were fixed with 2% formalin for 15 minutes at RT and permeabilized with a mAb (4ug/400ul) containing buffer (PBS, 0.2% BSA, 0.2% saponin) for 1 hour. FC: RAW264.7 cells were fixed for 15 minutes with 4% formalin and permeabilized (PBS, 0.5%BSA, 0,5% saponin) at RT. IHC-Paraffin: paraffin embedded tissues 5um sections were made. After antigen retrieval (0.01mol/l, pH6 sodium citrate) and quenching of endogenous peroxidase, sections were blocked with 0.5% ovalbumin and 0.1% gelatin for 20 minutes at RT. Sections were incubated with 5G5 for 1 hour at 37C. WB: reduced lysates were resolved by 10% SDS-PAGE and blotted on nitrocellulose. After blocking with 5% skimmed milk TLR9 was detected with 2 ug/ml 5G5 |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The TLR9 Antibody (5G5) from Novus Biologicals is a mouse monoclonal antibody to TLR9. This antibody reacts with mouse, canine. The TLR9 Antibody (5G5) has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. |
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| Immunogen | Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc |
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| Other Names | CD289, CD289 antigen, toll-like receptor 9 |
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| Gene, Accession # | TLR9, Gene ID: 54106, SwissProt: Q9NR96 |
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| Catalog # | NBP1-51156 |
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| Price | |
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| Order / More Info | TLR9 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | n/a |
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