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| Product Name | alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Activity Kit (Color) |
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| Description | a-ketoglutarate Dehydrogenase (a-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). a-KGDH converts a-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer's disease. Recent studies show that a-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. The a-KGDH assay kit provides a quick and easy way for monitoring a-KGDH activity in various samples. In the assay, a-KGDH converts a-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect a-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples. Sample types: adherent cell culture, animal tissues, heart, liver, muscle, purified mitochondria, suspension cell culture |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of a-Ketoglutarate dehydrogenase activity in various tissues/cellsAnalysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K617 |
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| Order / More Info | alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Activity Kit (Color) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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