产品详情 |
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Antigenic Specificity | HSP27 |
Clone | G3.1 |
Host Species | Mouse |
Reactive Species | human |
Isotype | IgG1 kappa |
Format | Protein A purified |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot. This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting, immunoprecipitation, ELISA, immunohistochemistry and immunocytochemistry. The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma. Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104. |
Immunogen | This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein. |
Other Names | mouse anti-HSP27 antibody, mouse anti-Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody |
Gene, Accession # | HSPB1, Gene ID: 3315, NCBI: 17647521, UniProt: P04792 |
Catalog # | 200-301-243 |
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Order / More Info | HSP27 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Ines Moretti-Rojas, et. al. (1988) A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res. and Treat., 11: 155-163.McGuire, S.E. et. al. (1989) Chromasomal Assignment of Human 27-kDa Heat Shock Protein Gene Family. Somatic Cell and Molecular Genetics, 15: 167-171.Edwards, D.P., et. al. (1981) Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Breast Cancer Res. Treat., 1: 209-223. |
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