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| Antigenic Specificity | Sheep IgG |
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| Clone | eB270 |
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| Host Species | Mouse |
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| Reactive Species | sheep |
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| Isotype | n/a |
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| Format | peroxidase (HRP) conjugate |
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| Size | 200 µL |
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| Concentration | 1000X (see application note, titrated reagent enough for 80 blots) |
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| Applications | Western Blot. Sheep IgG TrueBlot® is provided as 1000X solution. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-8815-33 (200µL) will yield enough reagent for 80 blots.Note that there are three key procedural considerations:1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Sheep TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum. Reactivity is observed against native Sheep IgG by both Western blot and ELISA. |
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| Immunogen | n/a |
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| Other Names | HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting |
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| Gene, Accession # | n/a |
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| Catalog # | 18-8815-33 |
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| Price | |
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| Order / More Info | Sheep IgG Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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