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| Antigenic Specificity | DYKDDDDK |
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| Clone | 29E4.G7 |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG2a kappa |
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| Format | peroxidase (HRP) conjugate |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Immunohistochemistry, Western Blot. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
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| Immunogen | This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling. |
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| Other Names | mouse anti-FLAG™ tag HRP conjugation, Enterokinase Cleavage Site (ECS), peroxidase conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
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| Gene, Accession # | n/a |
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| Catalog # | 200-303-383 |
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| Price | |
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| Order / More Info | DYKDDDDK Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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