| 产品详情 |
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| Antigenic Specificity | PAK 1/2/3 phospho T423 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Western Blot. This phospho specific polyclonal antibody was tested by ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immuno-precipitation. Lysates from Jurkat cells or PAK transfected cells may be used as a control.This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Researchers should determine optimal titers for other applications. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity purified antibody is directed against human PAK (p21-GTPase Activated Protein Kinase). The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pT423 of Human PAK 1/2/3. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA. Cross reactivity with PAK 1/2/3 from other species has not been determined. |
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| Immunogen | Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
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| Other Names | rabbit anti-PAK pT423 antibody, rabbit anti-PAK1/2/3 pT423 antibody, PAK1, PAK-1, PAK 1, Alpha PAK, p21-activated kinase 1, p65-PAK, PAK2, PAK-2, PAK 2, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Gamma PAK, PAK3, PAK-3, PAK 3, Beta PAK, CDC42/RAC effector kinase PAK-B, p21-activated kinase 3 |
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| Gene, Accession # | PAK1, Gene ID: 5058, NCBI: AAC50590.1, UniProt: Q13153 |
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| Catalog # | 600-401-413 |
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| Price | |
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| Order / More Info | PAK 1/2/3 phospho T423 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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