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Antigenic Specificity | Cul5, N-terminus |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human |
Isotype | n/a |
Format | delipidated & defibrinated |
Size | 100 µL |
Concentration | 85 mg/mL |
Applications | ELISA, Immunohistochemistry, Western Blot. This antibody reacts with human Cul5 by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using 293T, U2OS and others). This antibody also co-immunoprecipitates associated proteins. A 90.9 kDa band corresponding to human Cul5 is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 5. Cross reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross reactivity with other human cullins may occur. |
Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 4-18 of Human Cul5 (N-terminus) coupled to KLH. |
Other Names | rabbit anti-Cul5 Antibody, rabbit anti-Cullin5 antibody, VACM 1 antibody, VACM1 antibody, Vasopressin activated calcium mobilizing receptor 1 antibody, Vasopressin activated calcium mobilizing receptor antibody, CUL, Cullin |
Gene, Accession # | CUL5, Gene ID: 8065, NCBI: Q93034.4, UniProt: Q93034 |
Catalog # | 100-401-A05 |
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Order / More Info | Cul5, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541. |
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