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Antigenic Specificity | Cyclin B1 phospho S126, internal |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, rat, dog and chimpanzee, mouse, hamster. |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 2.01 mg/mL |
Applications | ELISA, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein. |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein. |
Other Names | rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1 |
Gene, Accession # | CCNB1, Gene ID: 891, UniProt: P14635 |
Catalog # | 600-401-464 |
Price | |
Order / More Info | Cyclin B1 phospho S126, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |