| 产品详情 |
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| Antigenic Specificity | Dab1, C-terminus |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse |
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| Isotype | n/a |
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| Format | delipidated & defibrinated |
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| Size | 100 µL |
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| Concentration | 75 mg/mL |
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| Applications | ELISA, Immunoprecipitation, Western Blot. Anti-Dab1 antibody is suitable for the detection of Dab1 by immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate. For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature. For immunoprecipitation use 1 µl of antiserum per 500 µg of brain lysate. Perform immunoprecipitation at 4°C for 2 h. For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 µg/ml aprotinin and 10 µg/ml leupeptin. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. This antibody is directed against Dab1 from mouse. Cross-reactivity with other species has not been determined. No reaction occurs with human or mouse Dab2. |
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| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555. |
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| Other Names | rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody |
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| Gene, Accession # | DAB1, Gene ID: 1600, NCBI: 33350928, UniProt: O75553 |
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| Catalog # | 100-401-225 |
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| Price | |
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| Order / More Info | Dab1, C-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Katyal, S., Gao, Z., Monckton, E., Glubrecht, D. and Godbout, R. (2007) Hierarchical Disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation. J.Mol. Bio. 368; 349-364.Sheldon, M., Rice, D.S. D'Arcangelo, G., Yoneshima, H., Nakajima, K., Mikoshiba, K., Howell, B.W., Cooper, J.A., Goldowitz, D. and Curran, T. (1997) Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice. Nature 389:730-733.Keshvara,L., Magdaleno,S., Benhayon,D. and Curran,T. (2002) Cyclin-dependent kinase 5 phosphorylates disabled 1 independently of Reelin signaling. J. Neurosci. 22 (12), 4869-4877. |
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