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| Antigenic Specificity | Pancreatic Islet Lysate |
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| Clone | polyclonal |
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| Host Species | Dog |
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| Reactive Species | porcine |
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| Isotype | n/a |
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| Format | IgG fraction |
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| Size | 500 µg |
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| Concentration | n/a |
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| Applications | This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum. Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates. |
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| Immunogen | Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells |
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| Other Names | IgG fraction of Anti-pancreatic islet lysate, Dog Anti-Pancreatic lysate (porcine pancreas), Anti-pancreatic lysate produced in dog, dog anti-Pancreatic Islet Lysate Antibody, canine anti-Pancreatic Islet Lysate Antibody |
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| Gene, Accession # | UniProt: P01300 |
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| Catalog # | 214-A01-118 |
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| Price | |
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| Order / More Info | Pancreatic Islet Lysate Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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