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| Product Name | IDH1, Recombinant, Human, FLAG-Tag |
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| Description | Purified (~80%). IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Description: Recombinant human IDH1 (aa 1-414, GenBank accesssion #NM_005896; UniProt O75874) with C-terminal FLAG tag, expressed in Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~47.6kD Specific Activity: ≥5600pmol/min/ug Assay Conditions: Reaction was conducted in 50mM Tris (pH 7.4), 10mM MgCl2, 200uM DL-isocitrate, and 200uM NADP. Incubation was carried out for |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | IDH1, Recombinant, Human, FLAG-Tag (Isocitrate Dehydrogenase [NADP] Cytoplasmic, IDH, Cytosolic NADP-isocitrate Dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) |
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| Gene, Accession, CAS # | Accession: O75874, SwissProt: O75874 |
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| Catalog # | 137303 |
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| Order / More Info | IDH1, Recombinant, Human, FLAG-Tag from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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