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| Product Name | Methyltransferase Activity Assay Kit (Colorimetric) |
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| Description | Methyltransferases are a diverse group of enzymes that methylate proteins, DNA, and small molecule targets. Accordingly, methyl transferase activity plays a critical role in modulating gene transcription, protein interactions, and signaling networks. Anomalous DNA methylation patterns have been consistently detected during cancer development as well as in many genetic disorders such as Fragile X Syndrome, ICF, and Rett Syndrome. The Methyltransferase Activity Assay Kit (Colorimetric) allows for kinetic evaluation of methyltransferase activity of purified enzymes and their inhibitors. The transfer of a methyl group from S-Adenosyl Methionine (SAM) cofactor to a corresponding substrate generates S-Adenosyl Homocysteine (SAH) as a product. SAH is detected by coupling the methyl transfer reaction to a multi-step enzymatic cascade, resulting in the generation of an intermediate that reacts with an OxiRed Probe. The reaction product exhibits a strong absorbance at 570 nm. The limit of quantification (L.O.Q) is 296 pmol of SAH generated per min per ml (296 µU/ml) of purified enzymes. Sample types: purified protein |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of Beta-Xylosidase activity in various biological samples and purified/crude enzyme preparations |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K767 |
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| Order / More Info | Methyltransferase Activity Assay Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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