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| Product Name | Beta-Lactamase Activity Kit (Colorimetric) |
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| Description | Beta-Lactamases (BetaLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. BetaLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as Beta-lactams. Antibiotics containing Beta-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. BetaLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing Beta-lactam antibiotic resistance. The Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which is directly proportional to the amount of BetaL activity. The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples. Sample types: bacterial cultures, fermentation media, food, mammal infected with beta-lactamase secreting bacteria saliva, mammal infected with beta-lactamase secreting bacteria serum, mammal infected with beta-lactamase secreting bacteria urine, milk |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of Beta-Lactamase activity in various biological samples. Analysis of Beta-Lactamase activity in pathological conditions. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K700 |
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| Order / More Info | Beta-Lactamase Activity Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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