| 产品详情 |
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| Product Name | TMB, Ultrasensitive |
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| Description | 3,3'5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 104 mo-11 cm-1) and diimine (E450 nm = 5.9 x 104 mol-1 cm-1) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBUS is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays. |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | 3,3',5,5'-Tetramethylbenzidine, TMBUS |
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| Gene, Accession, CAS # | CAS: 54827-17-7 |
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| Catalog # | LS-H2262 |
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| Price | |
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| Order / More Info | TMB, Ultrasensitive from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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