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Product Name | Akt Activity Kit |
Description | Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate. Phosphorylation of the GSK-3a can be analyzed by Western blot analysis using the phospho-GSK-3a specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected. Sample types: cell lysates, tissue homogenates |
Size | n/a |
Concentration | n/a |
Applications | This assay provides for the detection of AKT activity in various biological samples. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysates. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected. |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | LS-K98 |
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Order / More Info | Akt Activity Kit from LIFESPAN BIOSCIENCES INC. |
Product Specific References | n/a |
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