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| Product Name | Caspase 3 Apoptosis Detection, Colorimetric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. Kit Components (100 assays): C2087-20A: Cell Lysis Buffer C2087-20B: 2X Reaction Buffer C2087-20C: DEVD-pNA (4mM) C2087-20D: DTT (1M) C2087-20E: Dilution Buffer |
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| Size | 25 tests, 100 tests, 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-20 |
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| Order / More Info | Caspase 3 Apoptosis Detection, Colorimetric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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