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| Product Name | Caspase 9 Apoptosis Detection, Colorimetric BioAssay™ Kit (ICE-LAP6, Mch6, Apaf 3) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase 9 Apoptosis Detection, Colorimetric BioAssay™ Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity. Kit Components: 25 assays 100 assays C2088-10A: Cell Lysis Buffer 1 x 25ml 1 x 100ml C2088-10B: 2X Reaction Buffer 1 x 2ml 4 x 2ml C2088-10C: LEHD-pNA Substrate (4mM) 1 x 125ul 1 x 500ul C2088-10D: DTT (1M) 1 x 100ul 1 x 400ul C2088-10E: Dilution Buffer 1 x 25ml 1 x 100ml |
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| Size | 25 tests, 100 tests, 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2088-10 |
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| Order / More Info | Caspase 9 Apoptosis Detection, Colorimetric BioAssay™ Kit (ICE-LAP6, Mch6, Apaf 3) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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