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| Product Name | Caspase 3 Apoptosis Detection, Fluorometric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. Kit Components: 25 assays 100 assays C2087-10A Cell Lysis Buffer 25ml 100ml C2087-10B 2X Reaction Buffer 2ml 4 x 2ml C2087-10C DEVD-AFC Substrate (1mM) 125ul 500ul C2087-10D DTT (1M) 100ul 400ul |
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| Size | 25 tests, 100 tests, 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-10 |
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| Order / More Info | Caspase 3 Apoptosis Detection, Fluorometric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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