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| Product Name | Caspase 8 Apoptosis Detection, Fluorometric BioAssay™ Kit (FLICE, MASH, Mch5) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. USBio's Caspase-8 Apoptosis Detection Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity. Kit Contents: Assays 25 100 200 400 Cell Lysis Buffer 25ml 100ml 100ml 100ml 2X Reaction Buffer 2ml 4x2ml 16ml 32ml IETD-AFC (1mM) 0.125ml 0.5ml 2x.05ml 2x1ml DT (1M) 0.1ml 0.4ml 0.4ml 0.4ml |
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| Size | 25Assays, 100Assays, 200Assays |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-30 |
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| Order / More Info | Caspase 8 Apoptosis Detection, Fluorometric BioAssay™ Kit (FLICE, MASH, Mch5) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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