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| Product Name | Caspase 2, Apoptosis Detection, Fluorometric BioAssay™ Kit (ICH1, Nedd2) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by ICH-1 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity. Components: 25 assays 100 assays Cell Lysis Buffer 25ml 100ml 2X Reaction Buffer 2ml 4 x 2ml VDVAD-AFC Substrate (1mM) 125ul 500ul DTT (1M) 100ul 400ul Stability: Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4° |
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| Size | 25 tests, 100 tests, 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2086-75 |
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| Order / More Info | Caspase 2, Apoptosis Detection, Fluorometric BioAssay™ Kit (ICH1, Nedd2) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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