| 产品详情 |
| Edit |   |
|---|
| Product Name | Caspase-1/ICE Assay Kit (Fluorometric) |
|---|
| Description | Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (? max = 400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (? max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity. Sample types: cell lysates, tissue homogenates |
|---|
| Size | n/a |
|---|
| Concentration | n/a |
|---|
| Applications | This assay provides for the detection of Caspase-1/ICE in various biological sample types. This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity. |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | LS-K58 |
|---|
| Price | |
|---|
| Order / More Info | Caspase-1/ICE Assay Kit (Fluorometric) from LIFESPAN BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|