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| Product Name | Mutant Isocitrate Dehydrogenase (IDH) Activity Kit (Color) |
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| Description | In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) is an enzyme that catalyzes the decarboxylation of Isocitrate producing a-ketoglutarate and CO2. Mutations in both isoforms of IDH (IDH1 and IDH2) are commonly found in human cancers. The Mutant Isocitrate Dehydrogenase (Mutant IDH) causes a gain-of-function, which reduces its affinity for isocitrate and favors the conversion of a-ketoglutarate to D-2-Hydroxyglutarate. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. D2HG functions as an oncometabolite promoting cellular transformation. Recent studies show that increased Mutant IDH activity is associated with various cancers; therefore, detection of Mutant IDH activity is important for diagnosis and developing therapeutic strategies (e.g. Mutant IDH inhibitors). The Mutant IDH assay kit provides a quick and simple assay to monitor the Mutant IDH activity in biological samples. In the assay, Mutant IDH oxidizes NADPH into an NADP+, which decreases the absorbance at 340 nm. The assay is simple, sensitive, and can detect Mutant Isocitrate Dehydrogenase activity lower than 2 mU/ml in a variety of cancer samples. Sample types: adherent cancer cell cultures, cancer tissues, colon cancer tissue, lung cancer tissue, purified enzyme preparation, suspension cancer cell cultures |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | This assay has a detection sensitivity as low as 2 mU. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K771 |
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| Order / More Info | Mutant Isocitrate Dehydrogenase (IDH) Activity Kit (Color) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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