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| Product Name | Interleukin 4, Human (IL-4) BioAssay™ ELISpot Kit |
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| Description | Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-4. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-4 binds to antibody coated microtiter plate. microtiter plate coated with human IL-4 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-4. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-4 secreting location. Each individual spot representing an individual human IL-4 secreting cell. Background: Interleukin 4 (IL-4) was initially characterized as a B cell stimulatory factor (BSF-1) (1) and B-cell differentiation factor (BCDFγ) (2). It was subsequent |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | I8426-01A |
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| Order / More Info | Interleukin 4, Human (IL-4) BioAssay™ ELISpot Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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