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| Product Name | BRAF (V599E), Active, Recombinant, Human (v-raf murine sarcoma viral oncogene homolog B1) |
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| Description | Purified (~62%). Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), GenBank Accession No. NM_004333, a.a.417-end with Val-to-Glu mutation on a.a.600* and N-terminal GST tag, MW=69kD, expressed in Sf9 insect cells via a baculovirus expression system.*This mutation is often cited as V599E. Source: Recombinant protein corresponding to active human BRAF at N-terminal, fused to GST tag expressed in Sf9 insect cells. Molecular Weight: ~69kD Specific Activity: 150 pmol/min/ug Assay Conditions: Assay was performed in a kinase buffer containing 0.1mg/ml inactive MEK1 substrate and 10uM ATP. Reaction was done at 30°C for 30 minutes. ATP transferred was calculated using Kinase-Glo® (Promega). Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NM_004333 |
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| Catalog # | 171834 |
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| Order / More Info | BRAF (V599E), Active, Recombinant, Human (v-raf murine sarcoma viral oncogene homolog B1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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