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| Product Name | Human sTNF RII/TNFRSF1B Quantikine ELISA Kit |
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| Description | The Quantikine Human sTNF RII Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human sTNF RII in cell culture supernates, serum, plasma, or urine. It contains E. coli-expressed recombinant human sTNF RII as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human soluble Type II receptor for TNF, minus 53 amino acids from the proline-rich region of the protein just exterior to the transmembrane domain, with an apparent molecular weight of approximately 19.8 kDa. This immunoassay has been shown to accurately quantitate the recombinant soluble sTNF RII. Results obtained using natural sTNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural sTNF RII. Since the measurement of human sTNF RII by this immunoassay is insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples, (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF). Range: 7.80 - 500 pg/mL. Sensitivity: 2.3 pg/mL. Time: 3.5 hours or 4.5 hours |
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| Size | 1 pack (50 plates) |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | CD120b, CD120b antigen, Etanercept, p75 TNF receptor, p75TBPII, p75TNFR, soluble TNFR1B variant 1, TNFBRp80 TNF-alpha receptor, TNF-R2, TNFR2TNFR1B, TNF-R75, TNFR80, TNFRII, TNF-RII, TNF-R-II, TNFR-II, TNFRSF1B, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, tumor necrosis factor receptor superfamily member 1B, tumor necrosis factor receptor superfamily, member 1B, Tumor necrosis factor receptor type II |
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| Gene, Accession, CAS # | TNFRSF1B, Gene ID: 7133 |
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| Catalog # | PDRT200 |
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| Order / More Info | Human sTNF RII/TNFRSF1B Quantikine ELISA Kit from R&D SYSTEMS, INC. |
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| Product Specific References | PubMed: 10806045, 10817778, 11298120, 12204949, 12525606, 14738241, 14747646, 15602734, 15716832, 15772591, 15785303, 15942356, 16205317, 16644643, 16914126, 17010968, 17302897, 17419681, 17478621, 17572250, 18068948, 18558470, 18701106, 19212702, 19248103, 19806318, 21978728, 22308464, 22392817, 22617158, 22682876, 24473436, 24898299, 24923671, 25472811, 25860248, 25909710, 26636578, 27040725, 27208752, 27371811, 27482889, 27606615, 27907037, 27927628, 27936119, 27938334, 28066794, 28190687, 28208713, 28256549, 28351806, 29046481, 29691365, 30571380 |
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