| 产品详情 |
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| Antigenic Specificity | Gli1, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse, human |
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| Isotype | n/a |
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| Format | delipidated & defibrinated |
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| Size | 25 µL |
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| Concentration | 75 mg/ml |
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| Applications | ELISA, Immunohistochemistry, Western Blot. This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is observed against Mouse and Human Gli-1. Cross-reactivity with other species is likely but has not been determined. No reaction occurs with human or mouse Gli-2 or Gli-3. |
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| Immunogen | The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP). |
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| Other Names | rabbit anti-Gli-1 Antibody, rabbit anti-Gli1 Antibody, Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody |
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| Gene, Accession # | GLI1, Gene ID: 14632, NCBI: 4885279, UniProt: P47806 |
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| Catalog # | 100-401-223S |
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| Price | |
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| Order / More Info | Gli1, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Wetmore, C., Eberhardt, D. and Curran, T. (2000) The normal patched allele is expressed in medulloblastomas from mice with heterozygous germline mutation of patched. Cancer Res. 60(8): 2239-2246.Qualtrough D et al. (2004) Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment. Int J Cancer 110:831-7. Niemann C et al. (2003) Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis. Proc Natl Acad Sci U S A 100 Suppl 1:11873-80. |
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