| 产品详情 |
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| Product Name | Phosphine-biotin |
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| Description | Purity >95%. Labeling reactive centers of various types in cells with specific site-directed probes is a common method to explore both function and biochemical modification of proteins. The popular click chemistry method of protein labeling employs use of a reaction between an azido group and an alkyne on complimentary pairs of a specific reactive probe and a labeling agent (i.e. a tag) such as biotin or a fluorophore. The Staudinger ligation is an alternative to the click chemistry reaction in which a phosphine-labeled molecule reacts with an azido group on the opposing molecule of interest. Phosphine biotin is a labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction. Modified proteins can be detected using common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE. For example, phosphine-biotin has been used successfully in conjunction with DAz-1 or DAz-2 to label and detect sulfenic acid sites in proteins. |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | 2-(diphenylphosphino)-4-[21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1,17-dioxo-6,9,12-trioxa-2,16-diazaheneicos-1-yl]-benzoic acid, methyl ester, Phosphine-biotin |
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| Gene, Accession, CAS # | CAS: 608514-42-7 |
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| Catalog # | LS-H9388 |
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| Order / More Info | Phosphine-biotin from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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