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Antigenic Specificity | 6x-His Tag |
Clone | 3D5 |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG2b |
Format | alkaline phosphatase (AP) conjugate |
Size | 125 µl |
Concentration | n/a |
Applications | Western Blot: 1:1000-1:5000 |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | 6x-His Tag Monoclonal Antibody (3D5), AP. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant p |
Immunogen | n/a |
Other Names | His Tag; Histidine Tag; Histidine Epitope Tag; 6X His eptope tag; 6His tag; 6X His |
Gene, Accession # | n/a |
Catalog # | R932-25 |
Price | |
Order / More Info | 6x-His Tag Antibody from INVITROGEN ANTIBODIES |
Product Specific References | PubMed: 25358745, 26550690, 28693571 |