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| Product Name | ADP Assay Kit (Colorimetric/Fluorometric) |
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| Description | ADP is a product of ATP dephosphorylating and it can be rephosphorylated to ATP. Dehosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. This newly designed ADP Assay Kriot vpides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (OD = 570 nm) or fluorometric methods (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 µM ADP in biological samples. Sample types: biological fluids, cell culture media, cell lysates, tissue homogenates |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | This assay provides for the detection of ADP in various biological sample types. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K36 |
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| Order / More Info | ADP Assay Kit (Colorimetric/Fluorometric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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