| 产品详情 |
| Edit |   |
|---|
| Product Name | Cystathionine beta Synthase Inhibitor Screening Kit (Fluor) |
|---|
| Description | Cystathionine Beta Synthase (EC 4.2.1.22, CBetaS) is a Pyridoxal 5-Phosphate-dependent enzyme that uses cysteine and homocysteine as substrates to catalyze the formation of H2S and cystathionine. CBetaS is well-known for its role in human sulfur metabolism. The overexpression of CBetaS has been implicated in Down Syndrome, and also results in homocystinuria; or low levels of homocysteine in blood. Homocystinuria leads to a mutli-systemic disorder of the connective tissue, muscles, and central nervous system (CNS). For that reason, pharmacological inhibitors of human CBetaS present attractive therapeutic potential in order to restore baseline CBetaS activity and blood homocysteine levels. It has been shown that aminooxyacetic acid (AOAA) is an irreversible inhibitor that targets the PLP-binding site of CBetaS. That interaction inhibits the transaminase reaction that would yield cystathionine and H2S in wildtype conditions. The Cystathionine Beta Synthase Inhibitor Screening Kit is the first available kit that enables customers to screen inhibitors of CBetaS and quantify their therapeutic potential. The kit has a simple, easy-to-follow protocol and is high-throughput adaptable. In this kit, the product hydrogen sulfide reacts with the non-fluorescent azido-functional group to yield a fluorescent amino group (Ex/Em = 368/460 nm). In the presence of AOAA, CBetaS activity is inhibited, which subsequently abolishes the production of H2S and thus the fluorescent signal is reduced. |
|---|
| Size | n/a |
|---|
| Concentration | n/a |
|---|
| Applications | Screening and characterization of inhibitors of Cystathionine Beta Synthase (CBetaS) |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | LS-K630 |
|---|
| Price | |
|---|
| Order / More Info | Cystathionine beta Synthase Inhibitor Screening Kit (Fluor) from LIFESPAN BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|