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Product Name | Mix and Go Competent Cells Strain HB101 |
Description | HB101 strain chemically competent cells from Molecular Innovations are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of 3x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conv |
Size | 1 mL, 2 mL |
Concentration | n/a |
Applications | Blunt End Cloning, Colony PCR, Endpoint PCR, Low Copy PCR, Mutagenesis, TA Cloning |
Other Names | n/a |
Gene, Accession, CAS # | [HB101] |
Catalog # | MBS136042 |
Price | |
Order / More Info | Mix and Go Competent Cells Strain HB101 from MYBIOSOURCE INC. |
Product Specific References | n/a |
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