| 产品详情 |
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| Antigenic Specificity | Smad3, p Ser423, p Ser425 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse, rat, porcine, bovine, chicken, Xenopus, zebrafish |
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| Isotype | n/a |
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| Format | immunogen affinity purified |
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| Size | 0.1 mg |
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| Concentration | n/a |
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| Applications | Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Single Cell Western. Expect a band approximately 48 kDa in size corresponding - phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Smad3 [p Ser423, p Ser425] Antibody from Novus Biologicals is a rabbit polyclonal antibody to Smad3. This antibody reacts with human, mouse, rat, porcine, bovine, chicken, xenopus, zebrafish. The Smad3 [p Ser423, p Ser425] Antibody has been validated for the following applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Single Cell Western. This antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues |
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| Immunogen | Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. |
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| Other Names | DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, pSMAD, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3 |
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| Gene, Accession # | SMAD3, Gene ID: 4088, Accession: NC_000015.9, SwissProt: NC_000015.9 |
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| Catalog # | NBP1-77836 |
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| Price | |
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| Order / More Info | Smad3, p Ser423, p Ser425 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 18922473, 27356569 |
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