| 产品详情 |
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| Product Name | FluorGold Protein Dye |
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| Description | FluorGold Protein Dye is a fast and sensitive fluorescent dye for visualization and quantitation of proteins separated by 1-D or 2-D SDS-PAGE. It comes as a 100x stock solution that is simply diluted with water by the user to its working concentration. FluorGold Protein Dye is normally low fluorescent but emits strong fluorescence (bright golden color) as bound to proteins. The staining procedure is a simple two-step protocol (fix and stain) that can be completed in as little as 30 minutes. Gels to be stained are fixed with ethanol/acetic acid solution prior to staining with FluorGold Protein Dye solution. A destain step is not normally recommended, but may be employed to reduce background, simply by agitating the gel in water for 1-5 minutes. Gels stained with FluorGold Protein Dye fluorescent gel stain may be directly visualized with a variety of different UV-based fluorescence imaging systems. The maximum emission wavelength of protein-bound FluorGold Protein Dye is near 570 nm. FluorGold Protein Dye gives exceptional sensitivity and wide dynamic range for protein detection. The bound FluorGold Protein Dye dye is easily removed from the protein by immersing the gel in sufficient water, thus it is well compatible with subsequent enzymatic digestion and mass spectrometry for proteomics applications. Stained gels may be stored in stain solution in the dark at 2-8°C; imaging sensitivity might be moderately enhanced after 4°C storage of the stained gel. |
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| Size | 10 ml |
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| Concentration | n/a |
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| Applications | SDS-PAGE, WB |
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| Other Names | Fluoresent Dye, FluorGold DNA Dye, stain, promotions |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | orb90571 |
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| Order / More Info | FluorGold Protein Dye from BIORBYT LTD. |
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| Product Specific References | n/a |
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