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| Product Name | Caspase-3/CPP32 Assay Kit (Fluorometric) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (? max = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (? max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity. Sample types: cell lysates, tissue homogenates |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | This assay provides for the detection of Caspase-3/CPP32 in various biological sample types. This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3/CPP32 activity. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K60 |
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| Order / More Info | Caspase-3/CPP32 Assay Kit (Fluorometric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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