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| Product Name | Caspase-8/FLICE Assay Kit (Fluorometric) |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (? max = 400 nm); upon cleavage of the substrate by FLICE or related caspases, free AFC emits a yellow-green fluorescence (? max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity. Sample types: cell lysates, tissue homogenates |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | This assay provides for the detection of FLICE/Caspase-8 in various biological sample types. This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE/Caspase-8 activity. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K62 |
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| Order / More Info | Caspase-8/FLICE Assay Kit (Fluorometric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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