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| Product Name | Phospholipase D (PLD) Activity Kit (Colorimetric) |
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| Description | Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. The Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples. Sample types: adherent cell culture, animal tissues, brain, kidney, liver, suspension cell culture |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of PLD activity in various tissues/cells. Analyzing intracellular signalingScreening of PLD inhibitors |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K650 |
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| Order / More Info | Phospholipase D (PLD) Activity Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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