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| Product Name | Coelenterazine 400a |
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| Description | Purity >95%. Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins. The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum = 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green fluorescent protein (GFP), in bioluminescent resonance energy transfer (BRET) studies. Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by Renilla luciferase (Rluc). It is used in BRET2 protocols, whereas coelenterazine h, which has a 475 nm Rluc emission peak, is used in BRET1 protocols. The emission peak of coelenterazine 400a is commonly paired with class 1 and 3 GFPs, including GFP2 and GFP10. BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling. |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | 6-phenyl-2,8-bis (phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one, DeepBlueC™ |
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| Gene, Accession, CAS # | CAS: 70217-82-2 |
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| Catalog # | LS-H7214 |
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| Order / More Info | Coelenterazine 400a from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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