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| Product Name | Interleukin 4, Human (IL-4) BioAssay™ ELISA Kit |
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| Description | Principle: This IL-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-4 and incubated. IL-4 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be sandwiched by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-4 and other components of the sample. In order to quantitate the amount of IL-4 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | I8426-01 |
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| Order / More Info | Interleukin 4, Human (IL-4) BioAssay™ ELISA Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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