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| Product Name | Interleukin 6, Human (IL-6), BioAssay™ ELISpot Kit |
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| Description | Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-6. Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-6 binds to antibody coated microtiter plate. microtiter plate coated with human IL-6 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-6. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-6 secreting location. Each individual spot representing an individual human IL-6 secreting cell. Background: Interleukin 6 (IL-6) is a multifunctional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. (1) Syno |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | I8428-04L |
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| Price | |
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| Order / More Info | Interleukin 6, Human (IL-6), BioAssay™ ELISpot Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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